Fig. 6. MCM induces microglia phagocytosis via H1 receptor. A) Representative images illustrate latex microbeads phagocytosis of iba1-positive (red) microglial cells in non-treated cells (Control) and treated cells with, MCM, histamine 100 µM (HIS 100) and ATP 100 µM (ATP 100). B) Potentiation of phagocytosis by LPS, MCM, HIS 4 and HIS 100 at 48 h. No effect is induced by ATP 10 and ATP 100. C) Linear relation between iba1 intensity and beads per cell. D) Relationship between area and beads per cell. E) Phagocytosis induced by histamine 100 µM (HIS 100) is inhibited by cetirizine (H1R antagonist) but not by ranitidine (H2R antagonist), carcinine (H3R antagonist) or A943931 (H4R antagonist). F) Phagocytosis induced by MCM is inhibited by cetirizine but not by ranitidine, carcinine or A943931, similarly to histamine. Values (mean ± S.E.M) were computed from three independent culturing experiments. *p< 0.05; **p< 0.01; ***p<0.001, using the Student's t test. Scale bar 10 µm.